Rabbit polyclonal antibody to Claudin 3 (Phospho-Tyr219)
Specificity
The antibody detects endogenous levels of Claudin 3 only when phosphorylated at tyrosine 219.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 219 (R-K-D-Y(p)-V) derived from Human Claudin 3.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
C7orf1, Claudin-3, CLDN3, Clostridium perfringens enterotoxin receptor 2, CPE-R 2, CPE-receptor 2, CPETR2, hRVP1, Rat ventral prostate.1 protein homolog
Western blot analysis of extracts from COLO cells treated with EGF using Claudin 3 (Phospho-Tyr219) Antibody #11791.The lane on the right is treated with the antigen-specific peptide.
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