Anti-Chat Antibody (A82508)

Name: Anti-Chat Antibody
Brand: Antibodies.com
SKU: A82508
Price: 495.0 USD
Availability: InStock

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$495

Goat polyclonal antibody to Chat for ELISA, WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 3-5 business days.

Specifications

Name

Anti-Chat Antibody

Description

Goat polyclonal antibody to Chat.

Applications

ELISA, WB, IHC

Dilutions

ELISA: 1:128,000, WB: 0.3-1µg/ml, IHC: 1:2,000

Reactivity

Mouse, Rat

Cross Reactivity

This antibody is not expected to cross react to Human CHAT.

Immunogen

Synthetic peptide corresponding to the C terminal region of mouse Chat.

Sequence

C-KERARGPTKPSNLD

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

75 kDa

Predicted MW

72.3kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CHOACTase, Choline acetylase, Choline O-acetyltransferase

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-Chat Antibody (A82508)

Chat expression in Mouse (A) and Rat (B) Brain lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Chat Antibody (A82508) at 1µg/ml and detected by chemiluminescence.

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Immunohistochemistry - Anti-Chat Antibody (A82508)

Chat expression in Mouse Forebrain analyzed by immunohistochemistry. Staining was performed with Anti-Chat Antibody (A82508). Tissue was cryosectioned after PFA perfusion fixation Note HRP-staining with Ni-DAB, after Biotin-SP-anti goat (IgG).

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