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Anti-CDKN2A/p16INK4aF Antibody (A8379)

Western Blot - Anti-CDKN2A/p16INK4aF Antibody (A8379) - Antibodies.com Western Blot - Anti-CDKN2A/p16INK4aF Antibody (A8379) - Antibodies.com Immunofluorescence - Anti-CDKN2A/p16INK4aF Antibody (A8379) - Antibodies.com
Knockout Validated
$485
Product Datasheet

Rabbit polyclonal antibody to CDKN2A/p16INK4aF for WB and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-CDKN2A/p16INK4aF Antibody
Description
Rabbit polyclonal antibody to CDKN2A/p16INK4aF.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-156 of human CDKN2A/p16INK4a (NP_000068.1).
Sequence
QVMMMGSARVAELLLLHGAEPNCADPATLTRPVHDAAREGFLDTLVVLHRAGARLDVRDAWGRLPVDLAEELGHRDVARYLRAAAGGTRGSNHARIDAAEGPSDIPD
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
16 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-CDKN2A/p16INK4aF Antibody (A8379) - Antibodies.comEnlarge Image

Western Blot - Anti-CDKN2A/p16INK4aF Antibody (A8379)

Western blot analysis of HeLa, using Anti-CDKN2A/p16INK4aF Antibody (A8379) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western Blot - Anti-CDKN2A/p16INK4aF Antibody (A8379) - Antibodies.comEnlarge Image

Western Blot - Anti-CDKN2A/p16INK4aF Antibody (A8379)

Western blot analysis of extracts from wild type(WT) and CDKN2A/p16INK4a knockout (KO) 293T(KO) cells, using Anti-CDKN2A/p16INK4aF Antibody (A8379) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunofluorescence - Anti-CDKN2A/p16INK4aF Antibody (A8379) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-CDKN2A/p16INK4aF Antibody (A8379)

Immunofluorescence analysis of HeLa cells using Anti-CDKN2A/p16INK4aF Antibody (A8379) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
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