Anti-Cdk8 Antibody (A14830)

Name: Anti-Cdk8 Antibody
Brand: Antibodies.com
SKU: A14830
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to Cdk8 for WB and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Knockout Validated Antibody

Specifications

Name

Anti-Cdk8 Antibody

Description

Rabbit polyclonal antibody to Cdk8.

Applications

WB, IP

Dilutions

WB: 1:500-1:2,000, IP: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 365-464 of human CDK8 (NP_001251.1).

Sequence

DDKGDKKNQQQQQGNNHTNGTGHPGNQDSSHTQGPPLKKVRVVPPTTTSGGLIMTSDYQRSNPHAAYPNPGPSTSQPQSSMGYSATSQQPPQYSHQTHRY

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

56 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Cell division protein kinase 8, Cyclin-dependent kinase 8, Mediator complex subunit CDK8, Mediator of RNA polymerase II transcription subunit CDK8, Protein kinase K35

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Cdk8 Antibody (A14830)

Western blot analysis of extracts of Jurkat cells, using Anti-Cdk8 Antibody (A14830) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Western Blot - Anti-Cdk8 Antibody (A14830)

Western blot analysis of extracts from normal (control) and CDK8 knockout (KO) HeLa cells, using Anti-Cdk8 Antibody (A14830) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Western Blot - Anti-Cdk8 Antibody (A14830)

Immunoprecipitation analysis of 200µg extracts of Jurkat cells using 3µg of Anti-Cdk8 Antibody (A14830). This Western blot was performed on the immunoprecipitate using Anti-Cdk8 Antibody (A14830) at a dilution of 1:1000.

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