Rabbit polyclonal antibody to CDK6 (phospho-Tyr13)
Specificity
The antibody detects endogenous level of CDK6 only when phosphorylated at tyrosine 13.
Applications
WB, IHC, IF
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of tyrosine 13 (Q-Q-Y(p)-E-C) derived from Human CDK6.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Predicted MW
36kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CDK6 (Phospho-Tyr13) Antibody #11542 (left) or the same antibody preincubated with blocking peptide (right).
![Western Blot - Anti-Cdk6 Antibody [ARC0224] (A308341) - Antibodies.com](https://cdn.antibodies.com/image/catalog/308/A308341_1.jpg?profile=product_alternative)
