Anti-Cdk6 Antibody (A13498)

Name: Anti-Cdk6 Antibody
Brand: Antibodies.com
SKU: A13498
Price: 345.0 USD
Availability: InStock

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$345

Rabbit polyclonal antibody to Cdk6 for WB, IHC, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-Cdk6 Antibody

Description

Rabbit polyclonal antibody to Cdk6.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50-1:100

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human CDK6 (NP_001250.1).

Sequence

MEKDGLCRADQQYECVAEIGEGAYGKVFKARDLKNGGRFVALKRVRVQTGEEGMPLSTIREVAVLRHLETFEHPNVVRLFDVCTVSRTDRETKLTLVFEHVDQDLTTYLDKVPEPGVPTETIKDMMFQLLRGLDFLHSHRVVHRDLKPQNILVTSSGQIKLADFGLARIYSFQMALTSVVVTLWYRAPEVLLQSSYATPVDLWSVGCIFAEMFRRKPLFRGSSDVDQLGKILDVIGLPGEEDWPRDVALPRQAFHSKSAQPIEKFVTDIDELGKDLLLKCLTFNPAKRISAYSALSHPYFQDLERCKENLDSHLPPSQNTSELNTA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

40 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CDKN6, Cell division protein kinase 6, Cyclin-dependent kinase 6, Serine/threonine-protein kinase PLSTIRE

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Cdk6 Antibody (A13498)

Western blot analysis of HeLa, using Anti-Cdk6 Antibody (A13498) at 1:900 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 30s.

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Immunohistochemistry - Anti-Cdk6 Antibody (A13498)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Cdk6 Antibody (A13498) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Western Blot - Anti-Cdk6 Antibody (A13498)

Immunoprecipitation analysis of 200µg extracts of Jurkat cells using 3µg of Anti-Cdk6 Antibody (A13498). This Western blot was performed on the immunoprecipitate using Anti-Cdk6 Antibody (A13498) at a dilution of 1:1000.

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