Western blot analysis of extracts of various cell lines, using Anti-CDK5 Antibody (A14937) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using Anti-CDK5 Antibody (A14937) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Western blot analysis of extracts from normal (control) and CDK5 knockout (KO) 293T cells, using Anti-CDK5 Antibody (A14937) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunoprecipitation analysis of 900µg extracts of A-549 cells using 3µg of Anti-CDK5 Antibody (A14937). This Western blot was performed on the immunoprecipitate using Anti-CDK5 Antibody (A14937) at a dilution of 1:1000.