Anti-CDK2 (phospho Thr160) Antibody (A16579)

Western blot analysis of C6, using Anti-CDK2 (phospho Thr160) Antibody (A16579) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-CDK2 (phospho Thr160) Antibody (A16579). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.