Anti-CDK1 Antibody [ARC50607] (A305462)

Name: Anti-CDK1 Antibody [ARC50607]
Brand: Antibodies.com
SKU: A305462
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC50607] antibody to CDK1 for WB, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

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Centrosome Marker

Specifications

Name

Anti-CDK1 Antibody [ARC50607]

Description

Rabbit monoclonal [ARC50607] antibody to CDK1.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 198-297 of human CDK1 (NP_001777.1).

Sequence

ATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC50607

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

34 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CDC2, CDC28A, CDK10, CDKN1, Cell division control protein 2 homolog, Cell division protein kinase 1, Cell division protein kinase 10, Cyclin-dependent kinase 1, Cyclin-dependent kinase 10, p34 protein kinase, P34CDC2, Serine/threonine-protein kinase PISSLRE

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Western Blot - Anti-CDK1 Antibody [ARC50607] (A305462)

Western blot analysis of extracts of various cell lines, using Anti-CDK1 Antibody [ARC50607] (A305462) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Western Blot - Anti-CDK1 Antibody [ARC50607] (A305462)

Western blot analysis of extracts of Mouse spleen, using Anti-CDK1 Antibody [ARC50607] (A305462) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Immunofluorescence - Anti-CDK1 Antibody [ARC50607] (A305462)

Immunofluorescence analysis of HeLa using Anti-CDK1 Antibody [ARC50607] (A305462) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-CDK1 Antibody [ARC50607] (A305462)

Immunofluorescence analysis of NIH/3T3 using Anti-CDK1 Antibody [ARC50607] (A305462) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).