Anti-CDK1 Antibody (A305460)

Name: Anti-CDK1 Antibody
Brand: Antibodies.com
SKU: A305460
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to CDK1 for WB, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

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Centrosome Marker

Specifications

Name

Anti-CDK1 Antibody

Description

Rabbit polyclonal antibody to CDK1.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IP: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 198-297 of human CDK1 (NP_001777.1).

Sequence

ATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CDC2, CDC28A, CDK10, CDKN1, Cell division control protein 2 homolog, Cell division protein kinase 1, Cell division protein kinase 10, Cyclin-dependent kinase 1, Cyclin-dependent kinase 10, p34 protein kinase, P34CDC2, Serine/threonine-protein kinase PISSLRE

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (7)

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Western Blot - Anti-CDK1 Antibody (A305460)

Western blot analysis of extracts of various cell lines, using Anti-CDK1 Antibody (A305460) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Western Blot - Anti-CDK1 Antibody (A305460)

Western blot analysis of extracts of Jurkat cells, using Anti-CDK1 Antibody (A305460) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Western Blot - Anti-CDK1 Antibody (A305460)

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Immunofluorescence - Anti-CDK1 Antibody (A305460)

Immunofluorescence analysis of C6 cells using Anti-CDK1 Antibody (A305460) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).