Anti-CD99 Antibody (A8988)

Name: Anti-CD99 Antibody
Brand: Antibodies.com
SKU: A8988
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to CD99 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-CD99 Antibody

Description

Rabbit polyclonal antibody to CD99.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 23-185 of human CD99 (NP_002405.1).

Sequence

DGGFDLSDALPDNENKKPTAIPKKPSAGDDFDLGDAVVDGENDDPRPPNPPKPMPNPNPNHPSSSGSFSDADLADGVSGGEGKGGSDGGGSHRKEGEEADAPGVIPGIVGAVVVAVAGAISSFIAYQKKKLCFKENAEQGEVDMESHRNANAEPAVQRTLLEK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

22 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

12E7, CD99 antigen, E2 antigen, MIC2, MIC2X, MIC2Y, Protein MIC2, T-cell surface glycoprotein E2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-CD99 Antibody (A8988)

Western blot analysis of extracts of various cell lines, using Anti-CD99 Antibody (A8988) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.

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Immunohistochemistry - Anti-CD99 Antibody (A8988)

Immunohistochemistry analysis of paraffin-embedded human islet using Anti-CD99 Antibody (A8988) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-CD99 Antibody (A8988)

Immunohistochemistry analysis of paraffin-embedded human testis using Anti-CD99 Antibody (A8988) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-CD99 Antibody (A8988)

Immunofluorescence analysis of Jurkat cells using Anti-CD99 Antibody (A8988) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).

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