This antibody recognizes CD99, an approximately 32 kDa sialoglycoprotein expressed on the surface of many cell types, with particularly strong expression on Ewing´s sarcoma and peripheral primitive neuroectodermal tumors. Within the hematopoietic system, CD99 is expressed on virtually all cell types except granulocytes.
Applications
Flow Cytometry, CyTOF
Dilutions
Flow Cytometry: 1-4 µg/ml.
Reactivity
Human
Immunogen
Human thymocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
3B2/TA8
Isotype
IgG2a
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
32 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Surface staining (mass cytometry) of human peripheral blood cells (after separation using Ficoll-Paque density gradient centrifugation) with Anti-CD99 Antibody (A85607). Gated on singlets.
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