Recombinant rabbit monoclonal [DM84] antibody to CD86.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Clone ID
DM84
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Anti-CD86 Antibody [DM84] - BSA and Azide free (A318623)
ELISA plate pre-coated by 2 µg/ml (100 µl/well) Recombinant Human CD86 Protein (Fc Chimera 6xHis Tag) (A318374) can bind Anti-CD86 Antibody [DM84] - Azide free (A318623) in a linear range of 1-100 µg/ml.
Expi 293 cell line transfected with irrelevant protein (A) and human B7-2 (B) were surface stained with Anti-CD86 Antibody [DM84] - Azide free (A318623) at 1 µg/ml followed by Anti-Rabbit IgG Antibody (Alexa 488).
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