Anti-CD86 Antibody [BU63] (PE) (A279904)

Anti-CD86 Antibody [BU63] (PE)

Mouse monoclonal [BU63] antibody to CD86 (PE).

This antibody recognises human CD86 also known as B7-2, a type I transmembrane protein expressed by monocytes and activated B cells. CD86 acts as a co-stimulaory molecule along with CD80 and is a ligand for CD28 and CTLA-4. CD86 is a member of the Immunoglobulin superfamily and carries an extracellular domain bearing both an Ig-v-like domain which contains the CTLA-4 binding site and an adjacent C2-like domain. CD86 plays an important role in co-stimulation of T cell proliferation , IL-2 production and in the primary immune response. Domain depletion epitope mapping indicates that the binding site of Mouse anti Human CD86,clone Bu63 is located within the Ig-v-like domain of human CD86. CD86 along with CD80 may be exploited as receptors for adenovirus entry into cells (Short et al. 2004 2006).

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Human

Human peripheral blood lymphocytes.

Mouse

Monoclonal

BU63

IgG1

Protein A affinity chromatography of tissue culture supernatant.

Reconstitution dependent.

Lyophilized

Reconstitute with 1 ml of distilled water.

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse anti Human CD86 antibody, clone Bu63 recognizes human CD86 also known as B7-2, a type I transmembrane protein expressed by monocytes and activated B cells (Engel et al. 1994). CD86 acts as a co-stimulaory molecule along with CD80 (Lanier et al. 1995) and is a ligand for CD28 and CTLA-4 (Azuma et al. 1993).CD86 is a member of the Immunoglobulin superfamily and carries an extracellular domain bearing both an Ig-v-like domain which contains the CTLA-4 binding site and an adjacent C2-like domain. CD86 plays an important role in co-stimulation of T cell proliferation (Freeman et al. 1993), IL-2 production (Ribot et al. 2012) and in the primary immune response (Schultze et al. 1996).Domain depletion epitope mapping indicates that the binding site of Mouse anti Human CD86,clone Bu63 is located within the Ig-v-like domain of human CD86 (Jeanin et al. 1997).CD86 along with CD80 may be exploited as receptors for adenovirus entry into cells (Short et al. 2004 2006).

Activation B7-2 antigen, B70, BU63, CD28LG2, CTLA-4 counter-receptor B7.2, FUN-1, T-lymphocyte activation antigen CD86

This product is for research use only. It is not intended for diagnostic or therapeutic use.