This antibody recognises human CD82, also known as C33 antigen, Metastasis suppressor Kangai-1, Suppressor of tumorigenicity 6 protein or Tetraspanin-27. CD82 is a 267 amino acid ~50-53 kDa multiple pass transmembrane glycoprotein and member of the tetraspanin family expressed by T cells, NK cells, monocytes, granulocytes and platelets.
Applications
Flow Cytometry, IHC-Fr, IF
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Human
Immunogen
CD82 transfected murine mastocytoma cell line P815.
Host
Mouse
Clonality
Monoclonal
Clone ID
B-L2
Isotype
IgG1
Conjugate
Unconjugated
Purification
Ion exchange chromatography of tissue culture supernatant.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Human CD82 antibody, clone B-L2 recognizes human CD82, also known as C33 antigen, Metastasis suppressor Kangai-1, Suppressor of tumorigenicity 6 protein or Tetraspanin-27. CD82 is a 267 amino acid ~50-53 kDa multiple pass transmembrane glycoprotein and member of the tetraspanin family expressed by T cells, NK cells, monocytes, granulocytes and platelets.