Rat monoclonal [YTC182.20] antibody to CD8 (FITC).
Specificity
This antibody recognises the human CD8 cell surface antigen, a ~30/32 kDa glycoprotein expressed by the cytotoxic/suppressor subset of T lymphocytes, and more weakly by NK cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells or cells or 100µl whole blood.
Reactivity
Human, Cynomolgus monkey, Rhesus Monkey
Immunogen
Mouse L cells transfected with human CD8 gene.
Host
Rat
Clonality
Monoclonal
Clone ID
YTC182.20
Isotype
IgG2b
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Rat anti Human CD8 antibody, clone YTC182.20 recognizes the human CD8 cell surface antigen, a ~30/32 kDa glycoprotein expressed by the cytotoxic/suppressor subset of T lymphocytes, and more weakly by NK cells.