This antibody recognises the human CD8 cell surface antigen, a ~30/32 kDa glycoprotein expressed by the cytotoxic/suppressor subset of T lymphocytes, and more weakly by NK cells.
Applications
Flow Cytometry, IHC-Fr, IF
Dilutions
Flow Cytometry: 1:100 - 1:200, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Human, Cynomolgus monkey, Rhesus Monkey
Immunogen
Mouse L cells transfected with human CD8 gene.
Host
Rat
Clonality
Monoclonal
Clone ID
YTC182.20
Isotype
IgG2b
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rat anti Human CD8 antibody, clone YTC182.20 recognizes the human CD8 cell surface antigen, a ~30/32 kDa glycoprotein expressed by the cytotoxic/suppressor subset of T lymphocytes, and more weakly by NK cells.