This antibody recognises the bovine CD8 antigen expressed by a subset of T lymphocytes. The antibody precipitates molecules of ~34 kDa and ~38 kDa under reducing conditions. Clone CC63 has been reported as being suitable for use on formalin dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Bovine, Sheep, Goat
Host
Mouse
Clonality
Monoclonal
Clone ID
CC63
Isotype
IgG2a
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Bovine CD8 antibody, clone CC63 reacts with the bovine CD8 antigen expressed by a subset of T lymphocytes. The antibody precipitates molecules of ~34 kDa and ~38 kDa under reducing conditions. Clone CC63 has been reported as being suitable for use on formalin dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques (Gutierrez et al. 1999).