This antibody recognises the ovine CD8 cell surface antigen, which is expressed by the cytotoxic/suppressor subset of T lymphocytes.Under reducing conditions, the antigens immunoprecipitated by Mouse anti Sheep CD8 antibody, clone 38.65 migrate at ~33 kDa and ~36 kDa.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Sheep, Bovine, Goat
Immunogen
Sheep efferent lymphocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
38.65
Isotype
IgG2a
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Sheep CD8 antibody, clone 38.65 recognizes the ovine CD8 cell surface antigen, which is expressed by the cytotoxic/suppressor subset of T lymphocytes.Under reducing conditions, the antigens immunoprecipitated by Mouse anti Sheep CD8 antibody, clone 38.65 migrate at ~33 kDa and ~36 kDa.