Anti-CD8 alpha Antibody [OX-8] - BSA and Azide free (A281501)

Anti-CD8 alpha Antibody [OX-8] - BSA and Azide free

Mouse monoclonal [OX-8] antibody to CD8 alpha.

This antibody recognises the rat CD8 alpha cell surface antigen, expressed by a subset of T lymphocytes, most thymocytes and the majority of NK cells. Mouse anti Rat CD8a, clone MRC OX-8 is suitable for use in in vitro blocking assays. Mouse anti Rat CD8a, clone MRC OX-8 reacts with paraffin-embedded material following PLP Fixation (periodate-lysine paraformaldehyde). Mouse anti Rat CD8a, clone MRC OX-8 is routinely tested in flow cytometry on rat splenocytes.

IHC-Fr, IHC-P, WB, Flow Cytometry, IP, Functional Assays

Flow Cytometry: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl. This product does not require protein digestion pre-treatment of paraffin embedded sections.This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.

Rat

Rat Thymocyte Membrane Glycoproteins.

Mouse

Monoclonal

OX-8

IgG1

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline.

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse anti Rat CD8a, clone MRC OX-8, recognizes the rat CD8 alpha cell surface antigen, expressed by a subset of T lymphocytes, most thymocytes and the majority of NK cells.Mouse anti Rat CD8a, clone MRC OX-8 is suitable for use in in vitro blocking assays (Popov et al.2001).Mouse anti Rat CD8a, clone MRC OX-8 reacts with paraffin-embedded material following PLP Fixation (periodate-lysine paraformaldehyde) (Whiteland et al. 1995).Mouse anti Rat CD8a, clone MRC OX-8 is routinely tested in flow cytometry on rat splenocytes.

CD8a, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2