Anti-CD8 alpha Antibody [KT15] (A280830)

Anti-CD8 alpha Antibody [KT15]

Rat monoclonal [KT15] antibody to CD8 alpha.

This antibody recognises the alpha chain of mouse CD8. CD8 is a heterodimeric protein composed of disulphide-linked CD8a and CD8ß chains that is expressed primarily on cytotoxic T-cells. CD8 functions in the interaction with MHC Class I-bearing targets and plays a role in T-cell-mediated killing (Nakauchi , H. et al., 1985 & Nakauchi, H. et al., 1987).Clone KT15 is reported to block T-cell-mediated cytotoxicity in in vitro assays (Zeis, M. et al., 2002).

Flow Cytometry, IHC-Fr, IF

Flow Cytometry: 1:50 for 0.25 mg, 25 µg, 1:100 for 0.1 mg - 1:100 for 0.25 mg, 25 µg, 1:200 for 0.1 mg, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Mouse

T cell clone, C6.

Rat

Monoclonal

KT15

IgG2a

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rat anti Mouse CD8a, clone KT15, recognizes the alpha chain of mouse CD8. CD8 is a heterodimeric protein composed of disulphide-linked CD8a and CD8ß chains that is expressed primarily on cytotoxic T-cells. CD8 functions in the interaction with MHC Class I-bearing targets and plays a role in T-cell-mediated killing (Nakauchi , H. et al., 1985 & Nakauchi, H. et al., 1987).Clone KT15 is reported to block T-cell-mediated cytotoxicity in in vitro assays (Zeis, M. et al., 2002).

CD8a, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2