Anti-CD8 alpha Antibody [Du CD8-1] (A280269)

Anti-CD8 alpha Antibody [Du CD8-1]

Mouse monoclonal [Du CD8-1] antibody to CD8 alpha.

This antibody recognises Pekin duck CD8 alpha (CD8a). CD8a is expressed by thymocytes, splenocytes, peripheral lymphoid cells and the vast majority of bursal cells.Most avian immunity research has been carried out on chickens and relatively little is known about the immune system of ducks, though there is a resemblance between the main lymphoid organs: the spleen, thymus and bursa of Fabricius. At the cellular level, like mammalian T cells, duck lymphocytes are responsive to phytohaemagglutinin.Double-staining with clone Du CD8-1 and MCA2480 Mouse anti Duck IgY light chain clone 14A3 revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+/14A3+ cells in duck bursa. Mouse anti Duck CD8 alpha antibody, clone Du CD8-1 has been shown to not react with Mallard (Anas platyrhynchos).

IHC-P, Flow Cytometry, IP

Flow Cytometry: 1 - 10 µg/ml, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Duck

This antibody does not cross react with Chicken

293T cells expressing Pekin Duck CD8 alpha.

Mouse

Monoclonal

Du CD8-1

IgG2b

Protein G affinity chromatography of ascites.

500 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Duck CD8 alpha antibody, clone Du CD8-1 recognizes Pekin duck CD8 alpha (CD8a). CD8a is expressed by thymocytes, splenocytes, peripheral lymphoid cells and the vast majority of bursal cells.Most avian immunity research has been carried out on chickens and relatively little is known about the immune system of ducks, though there is a resemblance between the main lymphoid organs: the spleen, thymus and bursa of Fabricius. At the cellular level, like mammalian T cells, duck lymphocytes are responsive to phytohaemagglutinin.Double-staining with clone Du CD8-1 and MCA2480 Mouse anti Duck IgY light chain clone 14A3 revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+/14A3+ cells in duck bursa (Kothlow et al. 1985). Mouse anti Duck CD8 alpha antibody, clone Du CD8-1 has been shown to not react with Mallard (Anas platyrhynchos).

CD8a, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2