This antibody recognises the canine CD8 alpha chain which is expressed by thymocytes, peripheral T cells in the blood and lymphoid organs. Mouse anti Dog CD8a clone CA9.JD3 has been reported to inhibit cytotoxic T lymphocyte function. CA9.JD3 immunoprecipitates a heterodimer of ~32 kDa and ~36 kDa (reduced) from canine Tumor cells and from thymocytes.
Applications
IHC-Fr, IP, Flow Cytometry
Dilutions
Flow Cytometry: Neat, IHC-Fr: Neat - 1:10, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Canine
Immunogen
Canine thymocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
CA9.JD3
Isotype
IgG2a
Conjugate
Unconjugated
Concentration
Lot Specific
Purity
Tissue Culture Supernatant
Product Form
Liquid
Formulation
Supplied in <0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Dog CD8 alpha antibody, clone CA9.JD3 recognizes the canine CD8 alpha chain which is expressed by thymocytes, peripheral T cells in the blood and lymphoid organs .Mouse anti Dog CD8a clone CA9.JD3 has been reported to inhibit cytotoxic T lymphocyte function (Cobbold et al. 1994). CA9.JD3 immunoprecipitates a heterodimer of ~32 kDa and ~36 kDa (reduced) from canine Tumor cells and from thymocytes.