Anti-CD8 alpha Antibody [11/295/33] (PE) (A278955)

Anti-CD8 alpha Antibody [11/295/33] (PE)

Mouse monoclonal [11/295/33] antibody to CD8 alpha (PE).

This antibody recognises an epitope on the alpha chain of porcine CD8. Clone 11/295/33 detects a different epitope from that bound by clone 76-2-11.In addition to thymocytes and cytotoxic T cells, CD8 alpha is expressed on porcine NK cells. Swine CD8 alpha+CD3- NK cells are divided into NKp46+ (CD335) and NKp46- cells. A further CD8 alpha dim/- NKp46 high NK cell population can be found in porcine spleen and liver (Mair et al. 2012, 2013). Pigs are also remarkable in having significant levels of peripheral CD4 CD8 double positive T cells (Saalmüller et al. 1987; Zuckermann 1999).

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Porcine

Porcine lymphocytes.

Mouse

Monoclonal

11/295/33

IgG2a

Protein A affinity chromatography of tissue culture supernatant.

Reconstitution dependent.

Lyophilized

Reconstitute with 1 ml of distilled water.

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light.

Mouse anti Pig CD8 alpha antibody, clone 11/295/33 recognizes an epitope on the alpha chain of porcine CD8. Clone 11/295/33 detects a different epitope from that bound by clone 76-2-11.In addition to thymocytes and cytotoxic T cells, CD8 alpha is expressed on porcine NK cells. Swine CD8 alpha+CD3- NK cells are divided into NKp46+ (CD335) and NKp46- cells. A further CD8 alpha dim/- NKp46 high NK cell population can be found in porcine spleen and liver (Mair et al. 2012, 2013). Pigs are also remarkable in having significant levels of peripheral CD4 CD8 double positive T cells (Saalmüller et al. 1987; Zuckermann 1999).

CD8a, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

This product is for research use only. It is not intended for diagnostic or therapeutic use.