Anti-CD8 alpha Antibody [11/295/33] (A278956)

Anti-CD8 alpha Antibody [11/295/33]

Mouse monoclonal [11/295/33] antibody to CD8 alpha.

This antibody recognises an epitope on the alpha chain of porcine CD8. Clone 11/295/33 detects a different epitope from that bound by clone 76-2-11.In addition to thymocytes and cytotoxic T cells, CD8 alpha is expressed on porcine NK cells. Swine CD8 alpha+CD3- NK cells are divided into NKp46+ (CD335) and NKp46- cells. A further CD8 alpha dim/- NKp46 high NK cell population can be found in porcine spleen and liver (Mair et al. 2012, 2013). Pigs are also remarkable in having significant levels of peripheral CD4 CD8 double positive T cells (Saalmüller et al. 1987; Zuckermann 1999).

Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Porcine

Porcine lymphocytes.

Mouse

Monoclonal

11/295/33

IgG2a

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Pig CD8 alpha antibody, clone 11/295/33 recognizes an epitope on the alpha chain of porcine CD8. Clone 11/295/33 detects a different epitope from that bound by clone 76-2-11.In addition to thymocytes and cytotoxic T cells, CD8 alpha is expressed on porcine NK cells. Swine CD8 alpha+CD3- NK cells are divided into NKp46+ (CD335) and NKp46- cells. A further CD8 alpha dim/- NKp46 high NK cell population can be found in porcine spleen and liver (Mair et al. 2012, 2013). Pigs are also remarkable in having significant levels of peripheral CD4 CD8 double positive T cells (Saalmüller et al. 1987; Zuckermann 1999).

CD8a, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2