This antibody interacts with extracellular domain of CD79a, a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests; Extracellular and Intracellular staining.
Reactivity
Human
Immunogen
IgM complex isolated from Daudi cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
ZL7.4
Isotype
IgG1
Light Chains
kappa
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
40-45 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
B-cell antigen receptor complex-associated protein alpha chain, Ig-alpha, IGA, MB-1 membrane glycoprotein, MB1, Membrane-bound immunoglobulin-associated protein, Surface IgM-associated protein
Flow cytometric analysis of human peripheral whole blood stained with Anti-CD79a Antibody [ZL7.4] (PE) (10µl reagent per 100µl of peripheral whole blood).
Flow cytometry multicolor surface staining of human lymphocytes stained using Anti-CD79a Antibody [ZL7.4] (PE) (10µl reagent per 100µl of peripheral whole blood) and Anti-CD3 Antibody [UCHT1] (APC) (10µl reagent per 100µl of peripheral whole blood).
Separation of human CD79a positive B cells (red-filled) from CD79a negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained with Anti-CD79a Antibody [ZL7.4] (PE) (10µl reagent per 100µl of peripheral whole blood).
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