This antibody recognises an epitope within the cytoplasmic domain of CD79a. CD79a, also known as mb-1, is a 45 kDa protein that is expressed by B lymphocytes during differentiation from early pre-B cell stage through to plasma cells. The CD79a molecule associates with CD79b (B29) to form a heterodimer that is non-covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex. The CD79a/CD79b heterodimers are also necessary for intracellular signaling following antigen-binding to surface immunoglobulin.
Applications
Flow Cytometry
Dilutions
FC 1: Neat, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl
Reactivity
Human, Mouse, Rabbit, Horse, Pig, Monkey, Rat, Bovine, Guinea Pig, Fallow deer, American Bison, Red deer, Ferret, Goat
Immunogen
Synthetic peptide corresponding to amino acids 202-216 of human mb-1.
Sequence
ISRGLQGTYQDVGSL
Host
Mouse
Clonality
Monoclonal
Clone ID
HM57
Isotype
IgG1
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Human CD79a antibody, clone HM57 recognizes an epitope within the cytoplasmic domain of CD79a. CD79a, also known as mb-1, is a 45 kDa protein that is expressed by B lymphocytes during differentiation from early pre-B cell stage through to plasma cells. The CD79a molecule associates with CD79b (B29) to form a heterodimer that is non-covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex. The CD79a/CD79b heterodimers are also necessary for intracellular signaling following antigen-binding to surface immunoglobulin.
Synonyms
B-cell antigen receptor complex-associated protein alpha chain, Ig-alpha, IGA, MB-1 membrane glycoprotein, MB1, Membrane-bound immunoglobulin-associated protein, Surface IgM-associated protein
