Anti-CD74 Antibody [PIN.1] (A304910)

Name: Anti-CD74 Antibody [PIN.1]
Brand: Antibodies.com
SKU: A304910
Price: 410.0 USD
Availability: InStock

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$410

Mouse monoclonal [PIN.1] antibody to CD74 for WB, IHC, ICC/IF, IP, Flow Cytometry and FACS.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-CD74 Antibody [PIN.1]

Description

Mouse monoclonal [PIN.1] antibody to CD74.

Specificity

This antibody detects protein doublet corresponding to the molecular mass of the p33 and p35 forms of human CD74.

Applications

WB, IHC, ICC/IF, IP, Flow Cytometry, FACS

Dilutions

WB: 1:1,000, IHC: 1:100, ICC/IF: 1:50

Reactivity

Human, Mouse

Immunogen

Human CD74 invariant chain synthetic peptide.

Host

Mouse

Clonality

Monoclonal

Clone ID

PIN.1

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~33-35 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.2, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DHLAG, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, Ii

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Immunofluorescence - Anti-CD74 Antibody [PIN.1] (A304910)

Immunocytochemistry/Immunofluorescence analysis of human HaCaT cells, fixed in cold 100% methanol for 10 minutes at -20°C, using Anti-CD74 Antibody [PIN.1] (A304910), at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Cytoplasmic Staining.

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Western Blot - Anti-CD74 Antibody [PIN.1] (A304910)

Western blot analysis of human N87 cell lysates showing detection of CD74 protein using Anti-CD74 Antibody [PIN.1] (A304910) at 1:1,000. Lysates treated with macrophage inhibitory factor (MIF).

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Immunohistochemistry - Anti-CD74 Antibody [PIN.1] (A304910)

Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-CD74 Antibody [PIN.1] (A304910) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Beautiful basal to suprabasal staining in epidermis, dermis, hair follicles and muscle.

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Immunofluorescence - Anti-CD74 Antibody [PIN.1] (A304910)

Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-CD74 Antibody [PIN.1] (A304910), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus. Endosome. Lysosome. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-CD74 Antibody. (C) Composite.

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