CD74 expression in Human Tonsil lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-CD74 Antibody (A82638) at 0.01µg/ml and detected by chemiluminescence.
CD74 expression in Human Spleen analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-CD74 Antibody (A82638) at 5µg/ml and revealed with alkaline phosphatase (AP).
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