Mouse monoclonal [AD2] antibody to CD73 (APC-Cyanine 7).
Specificity
This antibody recognizes CD73, a 70 kDa GPI-anchored 5´-nucleotidase expressed predominantly on the surface of T and B cell subsets, follicular dendritic cells, and endothelial cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human, Non-Human Primates
Immunogen
Unfortunately, this information is considered to be commercially sensitive.
Host
Mouse
Clonality
Monoclonal
Clone ID
AD2
Isotype
IgG1
Light Chains
kappa
Conjugate
APC-Cyanine 7
Excitation: 650nm, Emission: 774nm
Purification
This antibody is conjugated with tandem dye APC-Cyanine 7 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
70 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow cytometric analysis of human peripheral whole blood stained using Anti-CD73 Antibody [AD2] (APC-Cyanine 7) (4µl reagent per 100µl of peripheral whole blood).
Flow cytometric analysis of human lymphocytes using Anti-CD19 Antibody [LT19] (PE) (4µl reagent per 100µl of peripheral whole blood) and Anti-CD73 Antibody [AD2] (APC-Cyanine 7) (4µl reagent per 100µl of peripheral whole blood).
Separation of human CD73 positive B cells (red-filled) from CD19 negative CD73 negative lymphocytes (black-dashed) in flow cytometry analysis of human peripheral whole blood using Anti-CD73 Antibody [AD2] (APC-Cyanine 7) (4µl reagent per 100µl of peripheral whole blood).
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