Anti-CD68 Antibody [ED1] (A280320)

Anti-CD68 Antibody [ED1]

Mouse monoclonal [ED1] antibody to CD68.

This antibody recognises the rat ED1 antigen, a heavily glycosylated protein of ~90 -110 kDa, also known as rat CD68. The ED1 antigen is expressed on most macrophages populations, as well as on monocytes and is considered as a pan-macrophage marker in the rat. ED1 is expressed predominantly on the lysosomal membrane and lightly on the cell surface. The expression of ED1 antigen being predominantly cytoplasmic , flow cytometry results are improved by the use of a membrane permeabilization procedure, such as Leucoperm, prior to staining.

WB, IHC-P, IP, Flow Cytometry, IHC-Fr, IF

FC 1: 1:50 - 1:100, IHC-P 2: 1:100, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Rat, Bovine

This antibody does not cross react with Horse

Rat spleen cells.

Mouse

Monoclonal

ED1

IgG1

Protein A affinity chromatography of tissue culture supernatant.

500 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti rat CD68 antibody, clone ED1 recognizes the rat ED1 antigen, a heavily glycosylated protein of ~90 -110 kDa, also known as rat CD68 (Dijkstra et al. 1985).The ED1 antigen is expressed on most macrophages populations, as well as on monocytes and is considered as a pan-macrophage marker in the rat (Damoiseaux et al. 1994). ED1 is expressed predominantly on the lysosomal membrane and lightly on the cell surface (Dijkstra et al. 1985).The expression of ED1 antigen being predominantly cytoplasmic (Dijkstra et al. 1985), flow cytometry results are improved by the use of a membrane permeabilization procedure, such as Leucoperm, prior to staining.

Gp110, Macrosialin