Anti-CD63 Antibody [NKI/C3] (PE) (A251167)

Anti-CD63 Antibody [NKI/C3] (PE)

Mouse monoclonal [NKI/C3] antibody to CD63 (PE).

This antibody recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Flow Cytometry, IF

Flow Cytometry: 5 µl / million cells or 5 µl / 100 µl whole blood, IF: 1:50-1:100

Human, Mouse

Smooth plasma membrane fraction of MeWo cells.

Mouse

Monoclonal

IgG1

kappa

Protein A/G chromatography.

100 µg/ml

26 kDa (core protein) / 30-60 kDa (glycosylated)

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Store at 4-8°C. Antibody is stable for 24 months.

CD63 antigen, Granulophysin, LAMP-3, Limp1, Lysosomal-associated membrane protein 3, Lysosome integral membrane protein 1, Melanoma-associated antigen ME491, MLA1, OMA81H, TSPAN30

This product is for research use only. It is not intended for diagnostic or therapeutic use.