Mouse monoclonal [MEM-259] antibody to CD63 (PE-Cyanine 5).
Specificity
This antibody reacts with an extracellular / luminal epitope of CD63, a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes / macrophages, and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
HPB-ALL human T cell line.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-259
Isotype
IgG1
Conjugate
PE-Cyanine 5
Excitation: 496nm, Emission: 667nm
Purification
This antibody is conjugated with tandem dye PE-Cyanine 5 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
40-60 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
CD63 antigen, Granulophysin, LAMP-3, Limp1, Lysosomal-associated membrane protein 3, Lysosome integral membrane protein 1, Melanoma-associated antigen ME491, MLA1, OMA81H, TSPAN30