Anti-CD63 Antibody [MEM-259] (APC) (A281142)

Anti-CD63 Antibody [MEM-259] (APC)

Mouse monoclonal [MEM-259] antibody to CD63 (APC).

This antibody recognises the CD63 cell surface antigen, also known as granulophysin, lysozomal associated membrane protein 3 (LAMP-3), Melanoma-associated antigen ME491, Ocular melanoma-associated antigen or tetraspanin-30. CD63 is a 238 amino acid, tetraspanin glycoprotein of ~40-60 kDa.CD63 is expressed by monocytes, macrophages, endothelial cells where it is a component of Weibel-Palade bodies and by a variety of cell lines. It is located intracellularly in lysosomal granules of platelets, being translocated to the surface upon activation. It may therefore by useful as a marker of platelet activation. CD63 expression is also associated with melanoma development and expression is rapidly down-regulated by progesterone in endometrial tissues.

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl or 100µl whole blood

Human

HPB-ALL cell line.

Mouse

Monoclonal

MEM-259

IgG1

Protein A affinity chromatography of tissue culture supernatant.

100 µg/ml after reconstituiton.

Lyophilized

Reconstitute with 1 ml of distilled water

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse anti Human CD63 antibody, clone MEM-259 recognizes the CD63 cell surface antigen, also known as granulophysin, lysozomal associated membrane protein 3 (LAMP-3), Melanoma-associated antigen ME491, Ocular melanoma-associated antigen or tetraspanin-30. CD63 is a 238 amino acid, tetraspanin glycoprotein of ~40-60 kDa.CD63 is expressed by monocytes, macrophages, endothelial cells where it is a component of Weibel-Palade bodies (Vischer et al. 1993) and by a variety of cell lines. It is located intracellularly in lysosomal granules of platelets, being translocated to the surface upon activation. It may therefore by useful as a marker of platelet activation (Hamamoto et al. 1994). CD63 expression is also associated with melanoma development (Radford et al. 1997) and expression is rapidly down-regulated by progesterone in endometrial tissues (Okada et al. 1999).

CD63 antigen, Granulophysin, LAMP-3, Limp1, Lysosomal-associated membrane protein 3, Lysosome integral membrane protein 1, Melanoma-associated antigen ME491, MLA1, OMA81H, TSPAN30

This product is for research use only. It is not intended for diagnostic or therapeutic use.