This antibody recognises the bovine homologue of human CD63, a 237 amino acid multipass transmembrane glycoprotein and member of the tetraspanin TM4SF protein family with a predicted molecular weight of ~26 kDa. CD63 is also known as lysosome associated membrane glycoprotein 3 or LAMP-3. CD63 along with other TM4SF members including CD9, CD61 and CD151 can form specific interactions with phosphoinositide 4-kinase, suggesting a role for CD63 in the recruitment of phosphoinositide 4-kinase to specific membrane sites. CD63 is expressed on the cell surface of platelets and basophils, along with activated macrophages, monocytes and granulocytes. Mouse anti Bovine CD63, clone CC25 acts as a specific marker for bovine lysozomes and has been used for the identification and quantitation of phagosome-lysozome fusion in models of bacterial insult.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1:10 - 1:25, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Bovine
Immunogen
Bovine PBMC.
Host
Mouse
Clonality
Monoclonal
Clone ID
CC25
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Bovine CD63 antibody, clone CC25 recognizes the bovine homologue of human CD63, a 237 amino acid multipass transmembrane glycoprotein and member of the tetraspanin TM4SF protein family with a predicted molecular weight of ~26 kDa. CD63 is also known as lysosome associated membrane glycoprotein 3 or LAMP-3. CD63 along with other TM4SF members including CD9, CD61 and CD151 can form specific interactions with phosphoinositide 4-kinase, suggesting a role for CD63 in the recruitment of phosphoinositide 4-kinase to specific membrane sites (Yauch and Hemler 2000). CD63 is expressed on the cell surface of platelets and basophils, along with activated macrophages, monocytes and granulocytes.Mouse anti Bovine CD63, clone CC25 acts as a specific marker for bovine lysozomes and has been used for the identification and quantitation of phagosome-lysozome fusion in models of bacterial insult (Souza et al. 2013).
Synonyms
CD63 antigen, Granulophysin, LAMP-3, Limp1, Lysosomal-associated membrane protein 3, Lysosome integral membrane protein 1, Melanoma-associated antigen ME491, MLA1, OMA81H, TSPAN30