Mouse monoclonal [Psel.KO.2.7] antibody to CD62P (PE).
Specificity
This antibody recognises human P-Selectin. CD62P is a ~140 kDa surface antigen expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:5, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Human, Mouse, Horse, Bovine, Rat, Goat, Cat, Sheep
Immunogen
P-selectin transfected 300.19 cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
Psel.KO.2.7
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Pack Size of 25 Tests: Reconstitute with 0.25 ml of distilled water. Pack Size of 100 Tests: Reconstitute with 1 ml of distilled water
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti human CD62P, clone Psel.KO.2.7, recognizes human P-Selectin. CD62P is a ~140 kDa surface antigen expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.
Synonyms
CD62 antigen-like family member P, GMP-140, GMRP, Granule membrane protein 140, GRMP, LECAM3, Leukocyte-endothelial cell adhesion molecule 3, P-selectin, PADGEM, Platelet activation dependent granule-external membrane protein, SELP