Mouse monoclonal [Psel.KO.2.12] antibody to CD62P.
Specificity
This antibody recognises the CD62P cell surface antigen, a ~140 kDa glycoprotein also known as P-selectin.CD62P is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells. Mouse anti Human CD62P antibody, clone Psel.KO.2.12 inhibits P-selectin-dependent adhesion between HL60 cells and P-selectin transfected COS cells.
Applications
IP, Flow Cytometry, IHC-Fr
Dilutions
Flow Cytometry: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl.
Reactivity
Human, Goat, Rat, Sheep
Immunogen
CD62P transfected 300.19 cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
Psel.KO.2.12
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Human CD62P antibody, clone Psel.KO.2.12 recognizes the CD62P cell surface antigen, a ~140 kDa glycoprotein also known as P-selectin.CD62P is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells. Mouse anti Human CD62P antibody, clone Psel.KO.2.12 inhibits P-selectin-dependent adhesion between HL60 cells and P-selectin transfected COS cells (Massaguer et al. 2000).
Synonyms
CD62 antigen-like family member P, GMP-140, GMRP, Granule membrane protein 140, GRMP, LECAM3, Leukocyte-endothelial cell adhesion molecule 3, P-selectin, PADGEM, Platelet activation dependent granule-external membrane protein, SELP