Anti-CD62L Antibody [MEL-14] (PE) (A281090)

$515

Rat monoclonal [MEL-14] antibody to CD62L (PE) for Flow Cytometry.

Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.
Name
Anti-CD62L Antibody [MEL-14] (PE)
Description
Rat monoclonal [MEL-14] antibody to CD62L (PE).
Specificity
This antibody recognises mouse L-selectin. L-selectin is an adhesion molecule expressed on most T and B lymphocytes, neutrophils, monocytes and eosinophils, (Fink, P. et al., 1985).Pre-incubation of lymphocytes with MEL-14 completely and specifically blocks binding of lymphocytes to high endothelial venules (HEV) in vitro and the migration of lymphocytes to lymph nodes in vivo (Gallatin, W. et al. 1983). Removal of sodium azide is recommended prior to using this clone in functional assays.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:2, Use 10µl of the suggested working dilution to label 106 cells in 100µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).
Reactivity
Mouse
Immunogen
Mouse B cell lymphoma, 38C-14.
Host
Rat
Clonality
Monoclonal
Clone ID
MEL-14
Isotype
IgG2a
Conjugate

PE

Excitation: 565nm, Emission: 578nm

Purification
Protein G affinity chromatography of ascites.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rat anti mouse CD62L, clone MEL-14, recognizes mouse L-selectin. L-selectin is an adhesion molecule expressed on most T and B lymphocytes, neutrophils, monocytes and eosinophils, (Fink, P. et al., 1985).Pre-incubation of lymphocytes with MEL-14 completely and specifically blocks binding of lymphocytes to high endothelial venules (HEV) in vitro and the migration of lymphocytes to lymph nodes in vivo (Gallatin, W. et al. 1983). Removal of sodium azide is recommended prior to using this clone in functional assays.
Synonyms
CD62 antigen-like family member L, L-selectin, LAM-1, LECAM1, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, LNHR, LYAM1, Lymph node homing receptor, SELL
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Publishing research using Anti-CD62L Antibody [MEL-14] (PE) (A281090)? Please let us know so that we can list the citation on this page.

Alternative products to Anti-CD62L Antibody [MEL-14] (PE) (A281090)