Anti-CD62L Antibody [FMC46] (A280480)

Anti-CD62L Antibody [FMC46]

Mouse monoclonal [FMC46] antibody to CD62L.

This antibody recognises human CD62L, also known a L-selectin, a 74-95 kDa member of the selectin family of adhesion receptors, which acts as a ligand for both CD62P (P-selectin) and CD62E (E-selectin). Human CD62L is constitutively expressed on most leucocytes including monocytes, granulocytes, lymphocytes, NK cells, bone marrow myeloid progenitor cells and on a subset of thymocytes.CD62L plays an important role in leucocyte tethering and rolling on the endothelial cell surface and for the homing of naïve lymphocytes to lymph nodes and Peyers patches via HEV. Neutrophils require a constant supply of this molecule on the cell surface for migration into peripheral tissues and adhesion to activated endothelium at sites of inflammation, where CD62L is rapidly shed as soluble L-selectin, but surface expression still remains.The expression of CD62L is down regulated on lymphocytes and neutrophils by PMA stimulation.

IHC-P, IP, Flow Cytometry, IHC-Fr

Flow Cytometry: 1:50 - 1:100, IHC-P: 10 µg/ml, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Human, Bovine, Cynomolgus monkey, Rhesus Monkey, Dog

PHA stimulated lymphoblasts.

Mouse

Monoclonal

FMC46

IgG2b

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human CD62L antibody, clone FMC46 recognizes human CD62L, also known a L-selectin, a 74-95 kDa member of the selectin family of adhesion receptors, which acts as a ligand for both CD62P (P-selectin) and CD62E (E-selectin). Human CD62L is constitutively expressed on most leucocytes including monocytes, granulocytes, lymphocytes, NK cells, bone marrow myeloid progenitor cells and on a subset of thymocytes.CD62L plays an important role in leucocyte tethering and rolling on the endothelial cell surface and for the homing of naïve lymphocytes to lymph nodes and Peyers patches via HEV. Neutrophils require a constant supply of this molecule on the cell surface for migration into peripheral tissues and adhesion to activated endothelium at sites of inflammation, where CD62L is rapidly shed as soluble L-selectin, but surface expression still remains.The expression of CD62L is down regulated on lymphocytes and neutrophils by PMA stimulation.

CD62 antigen-like family member L, L-selectin, LAM-1, LECAM1, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, LNHR, LYAM1, Lymph node homing receptor, SELL