Anti-CD62L Antibody [CC32] (A280039)

Anti-CD62L Antibody [CC32]

Mouse monoclonal [CC32] antibody to CD62L.

This antibody recognises bovine L-selectin, also known as CD62L, Leukocyte-endothelial cell adhesion molecule 1, LECAM-1 or Lymph node homing receptor.Bovine CD62L is a 370 amino acid ~90 kDa, single pass type I transmembrane glycoprotein bearing a single C-type lectin domain, an EGF-like domain and two Sushi domains (UniProt: P98131). Immunoprecipitation of peripheral blood mononuclear cell lysates with Mouse anti Bovine CD62L antibody, clone CC32 reveals a molecule of ~90 kDa when run on polyacrylamide gels under reducing conditions, slightly larger than the murine and human CD62L homologues. Bovine CD62L is expressed on subpopulations of T-lymphocytes expressing CD2, CD4 and CD8. WC1 positive ?/d T cells also express CD62L as do a subpopulation of WC3+ve B-lymphocytes and all peripheral blood monocytes. Mouse anti Bovine CD62L antibody, clone CC32 has also been used successfully for the identification of CD62L on ovine peripheral blood cells by flow cytometry.

Flow Cytometry: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Bovine, Sheep

Bovine lymphocytes.

Mouse

Monoclonal

CC32

IgG1

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Bovine CD62L antibody, clone CC32 recognizes bovine L-selectin, also known as CD62L, Leukocyte-endothelial cell adhesion molecule 1, LECAM-1 or Lymph node homing receptor.Bovine CD62L is a 370 amino acid ~90 kDa, single pass type I transmembrane glycoprotein bearing a single C-type lectin domain, an EGF-like domain and two Sushi domains (UniProt: P98131). Immunoprecipitation of peripheral blood mononuclear cell lysates with Mouse anti Bovine CD62L antibody, clone CC32 reveals a molecule of ~90 kDa when run on polyacrylamide gels under reducing conditions, slightly larger than the murine and human CD62L homologues. Bovine CD62L is expressed on subpopulations of T-lymphocytes expressing CD2, CD4 and CD8. WC1 positive ?/d T cells also express CD62L as do a subpopulation of WC3+ve B-lymphocytes and all peripheral blood monocytes (Howard et al. 1992).Mouse anti Bovine CD62L antibody, clone CC32 has also been used successfully for the identification of CD62L on ovine peripheral blood cells by flow cytometry (Halliday et al. 2005).

CD62 antigen-like family member L, L-selectin, LAM-1, LECAM1, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, LNHR, LYAM1, Lymph node homing receptor, SELL