This antibody reacts with a well defined epitope (W40, R-53) on CD59, a 18-20 kDa GPI-anchored glycoprotein expressed on the surface of all hematopoietic cells - it is widely present on cells in all tissues. This antibody does not compete with MEM-43/5.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Thymocytes and T lymphocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-43
Isotype
IgG2a
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
18-20 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!