Recombinant Anti-CD59 Antibody [MACIF/7021R] (A278101)

Anti-CD59 Antibody [MACIF/7021R]

Recombinant rabbit monoclonal [MACIF/7021R] antibody to CD59.

This antibody recognizes human CD59, a 20kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein (Workshop VI; Code N-L036). CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 is widely distributed on cells in all tissues. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). This MAb recognizes CD59 transfected cells. It is useful for study on GPI-anchored proteins, PNH and CD59 functions.

IHC-P: 1-2 µg/ml

Human

This antibody does not cross react with Baboon or Horse.

Recombinant full-length human CD59 protein.

Rabbit

Monoclonal

MACIF/7021R

Protein A chromatography.

200 µg/ml

20 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-CD59 Antibody [MACIF/7021R] - BSA and Azide free (A278689).

1F5 antigen, 20 kDa homologous restriction factor, CD59 glycoprotein, HRF-20, HRF20, MAC-inhibitory protein, MAC-IP, MACIF, MEM43 antigen, Membrane attack complex inhibition factor, MIC11, MIN1, MIN2, MIN3, MSK21