Anti-CD55 Antibody [BRIC216] (A279858)

Anti-CD55 Antibody [BRIC216]

Mouse monoclonal [BRIC216] antibody to CD55.

This antibody recognises the CD55 antigen, a ~70 kDa glycoprotein also known as Decay Accelerating Factor (DAF). CD55 is distributed on erythrocytes and other circulating blood cells and also on cells in non-haemopoietic tissue particularly epithelium and endothelium. CD55 is also expressed at the foetal-maternal interfaces in placenta.CD55 has reduced expression on individuals with paroxysmal nocturnal haemoglobinuria. recognises the consensus region 3 of the DAF molecule, which contains the functional site, and the antibody blocks the function of DAF.

WB, Functional Assays, Flow Cytometry, IP

Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Human

Human fibroblast cell line.

Mouse

Monoclonal

BRIC216

IgG1

1 mg/ml

Liquid

Supplied in TRIS Buffered Saline with <0.1% Sodium Azide and =100mM glycine.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human CD55 antibody, clone BRIC216 recognizes the CD55 antigen, a ~70 kDa glycoprotein also known as Decay Accelerating Factor (DAF). CD55 is distributed on erythrocytes and other circulating blood cells and also on cells in non-haemopoietic tissue particularly epithelium and endothelium. CD55 is also expressed at the foetal-maternal interfaces in placenta.CD55 has reduced expression on individuals with paroxysmal nocturnal haemoglobinuria. Mouse anti Human CD55 antibody, clone BRIC216 has a functional binding affinity to erythrocytes of 8.7 x 107 M-1. The antigen is pronase and trypsin resistant and chymotrypsin sensitive. Mouse anti Human CD55 antibody, clone BRIC216 recognizes the consensus region 3 of the DAF molecule, which contains the functional site, and the antibody blocks the function of DAF.

Complement decay-accelerating factor, CR, DAF