This antibody reacts with CD52, a 21-28 kDa extracellular glycoprotein containing a large N-linked carbohydrate moiety. Mature CD52 molecule is actually much smaller (approx. 8-9 kDa). CD52 is expressed at high levels on lymphocytes, monocytes / macrophages, and in male reproductive tract.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Human tonsil.
Host
Mouse
Clonality
Monoclonal
Clone ID
HI186
Isotype
IgG2b
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
21-28 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
Cambridge pathology 1 antigen, CAMPATH-1 antigen, CDW52, Epididymal secretory protein E5, He5, Human epididymis-specific protein 5