This antibody reacts with CD52, a 21-28 kDa extracellular glycoprotein containing a large N-linked carbohydrate moiety. Mature CD52 molecule is actually much smaller (approx. 8-9 kDa). CD52 is expressed at high levels on lymphocytes, monocytes / macrophages, and in male reproductive tract.
Applications
Flow Cytometry, IHC-P
Dilutions
Flow Cytometry: 2 µg/ml.
Reactivity
Human
Immunogen
Human tonsil.
Host
Mouse
Clonality
Monoclonal
Clone ID
HI186
Isotype
IgG2b
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
21-28 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Cambridge pathology 1 antigen, CAMPATH-1 antigen, CDW52, Epididymal secretory protein E5, He5, Human epididymis-specific protein 5
Flow cytometry analysis of CD52 in patients suffering with Acute Lymphoblastic Leukemia (Anti-CD52 Antibody (A85766). Detection by Goat Anti-Mouse IgG2b Antibody PE). CD52 negative pro T ALL (gate leukemic cells).