Anti-CD52 Antibody [11/305/44] (A278958)

Anti-CD52 Antibody [11/305/44]

Mouse monoclonal [11/305/44] antibody to CD52.

This antibody recognises the porcine homologue of human CD52, a ~19 kDa antigen expressed by mature lymphocytes, monocytes and dendritic cells. Mouse anti Pig CD52, clone 11/305/44 was originally clustered at the 1st International Swine Cluster of Differentiation Workshop held in 1992 as SWC1. SWC1 is the porcine orthologue to human CD52, expressed by most leucocytes including resting T-cells, monocytes and granulocytes, but is not expressed by B-cells, erythrocytes or platelets &. Porcine CD52, expressed at very much higher levels on monocytes than mature macrophages, and SWC9, expressed exclusively on mature tissue macrophages, have been used as markers of monocyte-macrophage differentiation &.

IHC-P, Flow Cytometry

Flow Cytometry: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Porcine

Porcine thymocytes.

Mouse

Monoclonal

11/305/44

IgG2b

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Pig CD52, clone 11/305/44 is a monoclonal antibody recognizing the porcine homologue of human CD52, a ~19 kDa antigen expressed by mature lymphocytes, monocytes and dendritic cells.Mouse anti Pig CD52, clone 11/305/44 was originally clustered at the 1st International Swine Cluster of Differentiation Workshop held in 1992 as SWC1 (Lunney et al.1994). SWC1 is the porcine orthologue to human CD52, expressed by most leucocytes including resting T-cells, monocytes and granulocytes, but is not expressed by B-cells, erythrocytes or platelets (Piriou-Guyzlack et al. 2008) & (Leitner et al. 2012). Porcine CD52, expressed at very much higher levels on monocytes than mature macrophages, and SWC9, expressed exclusively on mature tissue macrophages, have been used as markers of monocyte-macrophage differentiation (Sanchez et al. 1999) & (McCollough et al. 1999).

Cambridge pathology 1 antigen, CAMPATH-1 antigen, CDW52, Epididymal secretory protein E5, He5, Human epididymis-specific protein 5