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Anti-CD5 (phospho Tyr453) Antibody (A93556)

Western Blot - Anti-CD5 (phospho Tyr453) Antibody (A0849) - Antibodies.com Immunofluorescence - Anti-CD5 (phospho Tyr453) Antibody (A0849) - Antibodies.com Western Blot - Anti-CD5 (phospho Tyr453) Antibody (A0849) - Antibodies.com
$190
Product Datasheet

Rabbit polyclonal antibody to CD5 (phospho Tyr453) for WB, IF and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-CD5 (phospho Tyr453) Antibody
Description
Rabbit polyclonal antibody to CD5 (phospho Tyr453).
Specificity
This antibody detects endogenous levels of CD5 only when phosphorylated at Tyr453.
Applications
WB, IF, ELISA
Dilutions
WB: 1:500-1:1000, IF: 1:100-1:500, ELISA: 1:20000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human CD5 around the phosphorylation site of Tyr453 (amino acids 421-470).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
54kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
LEU1, Lymphocyte antigen T1/Leu-1, T-cell surface glycoprotein CD5
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-CD5 (phospho Tyr453) Antibody (A0849) - Antibodies.comEnlarge Image

Western Blot - Anti-CD5 (phospho Tyr453) Antibody (A93556)

Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30' using Anti-CD5 (phospho Tyr453) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunofluorescence - Anti-CD5 (phospho Tyr453) Antibody (A0849) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-CD5 (phospho Tyr453) Antibody (A93556)

Immunofluorescence analysis of HepG2 cells using Anti-CD5 (phospho Tyr453) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Western Blot - Anti-CD5 (phospho Tyr453) Antibody (A0849) - Antibodies.comEnlarge Image

Western Blot - Anti-CD5 (phospho Tyr453) Antibody (A93556)

Western blot analysis of 293 cells using Anti-CD5 (phospho Tyr453) Antibody.
Publishing research using Anti-CD5 (phospho Tyr453) Antibody (A93556)? Please let us know so that we can list the citation on this page.

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