Anti-CD5 Antibody [1H6/8] (FITC) (A279115)

Anti-CD5 Antibody [1H6/8] (FITC)

Mouse monoclonal [1H6/8] antibody to CD5 (FITC).

This antibody recognises pig CD5. Clone 1H6/8 was clustered as CD5a at the Second International Swine CD antigen Workshop , based on its cellular distribution, ability to completely inhibit the binding of monoclonal antibody clone b53b7, the internal control for the second workshop and the immunoprecipitation of the same 55 kDa + 60 kDa doublet as the control antibody. Porcine CD5 is expressed by all circulating peripheral blood T lymphocytes and some B cells. CD5 is not expressed by porcine ?/d T cells and can hence be used for the discrimination between NK cells (CD4- CD8+ CD5-) and MHC-restricted cytotoxic T cells (CD4- CD8+ CD5+). No reactivity of Mouse anti Pig CD5 antibody, clone 1H6/8 was noted with canine, bovine, equine or human tissue samples (Doménech et al. 2003).

WB, IP, Flow Cytometry

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to 1x10⁶ cells in 100µl

Porcine

Con A / PMA activated porcine peripheral blood cells.

Mouse

Monoclonal

1H6/8

IgG2a

Protein G affinity chromatography of tissue culture supernatant.

100 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Mouse anti Pig CD5 antibody, clone 1H6/8 recognizes pig CD5. Clone 1H6/8 was clustered as CD5a at the Second International Swine CD antigen Workshop (Saalmuller et al. 1998), based on its cellular distribution, ability to completely inhibit the binding of monoclonal antibody clone b53b7, the internal control for the second workshop and the immunoprecipitation of the same 55 kDa + 60 kDa doublet as the control antibody (Pescowitz et al. 1998). Porcine CD5 is expressed by all circulating peripheral blood T lymphocytes and some B cells. CD5 is not expressed by porcine ?/d T cells (Saalmuller et al. 1994) and can hence be used for the discrimination between NK cells (CD4- CD8+ CD5-) and MHC-restricted cytotoxic T cells (CD4- CD8+ CD5+). No reactivity of Mouse anti Pig CD5 antibody, clone 1H6/8 was noted with canine, bovine, equine or human tissue samples (Doménech et al. 2003).

LEU1, Lymphocyte antigen T1/Leu-1, T-cell surface glycoprotein CD5

This product is for research use only. It is not intended for diagnostic or therapeutic use.