Recombinant Anti-CD48 Antibody [DM43] - BSA and Azide free (A318652)

Name: Anti-CD48 Antibody [DM43] - BSA and Azide free
Brand: Antibodies.com
SKU: A318652
Price: 690.0 USD
Availability: InStock

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$690

Recombinant rabbit monoclonal [DM43] antibody to CD48 for ELISA and Flow Cytometry.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

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Recombinant Antibody

Specifications

Name

Anti-CD48 Antibody [DM43] - BSA and Azide free

Description

Recombinant rabbit monoclonal [DM43] antibody to CD48.

Applications

ELISA, Flow Cytometry

Dilutions

ELISA: 1:5,000-10,000, Flow Cytometry: 1:100

Reactivity

Human

Host

Rabbit

Clonality

Monoclonal

Clone ID

DM43

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity chromatography.

Concentration

Reconstitution dependent.

Product Form

Lyophilized

Reconstitution

Reconstitute with distilled sterile water.

Formulation

Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.

General Notes

Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.

Synonyms

B-lymphocyte activation marker BLAST-1, BCM1, BCM1 surface antigen, BLAST1, CD48 antigen, Leukocyte antigen MEM-102, Signaling lymphocytic activation molecule 2, SLAM family member 2, SLAMF2, TCT.1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Flow Cytometry - Anti-CD48 Antibody [DM43] - BSA and Azide free (A318652)

Expi 293 cell line was transfected with irrelevant protein (A) and human CD48 (B) were surface stained with Anti-CD48 Antibody [DM43] - BSA and Azide free (A318652) at 1µg/ml followed by Anti-Rabbit IgG Antibody (Alexa 488).

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Flow Cytometry - Anti-CD48 Antibody [DM43] - BSA and Azide free (A318652)

Flow cytometry data of serially titrated Anti-CD48 Antibody [DM43] - BSA and Azide free (A318652) on H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

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Flow Cytometry - Anti-CD48 Antibody [DM43] - BSA and Azide free (A318652)

Affinity ranking of different Rabbit Anti-CD48 Monoclonal Antibody clones by titration of different concentrations onto H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

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Flow Cytometry - Anti-CD48 Antibody [DM43] - BSA and Azide free (A318652)

Flow cytometry analysis of antigen binding of Anti-CD48 Antibody [DM43] - BSA and Azide free (A318652). (A) Anti-CD48 Antibody [DM43] - BSA and Azide free (A318652) does not bind to 293T cells that do not express CD48. (B) A clear peak shift of Anti-CD48 Antibody [DM43] - BSA and Azide free (A318652) was seen compared to the control when incubated with CD48-expressing Raji cells, indicating strong binding of Anti-CD48 Antibody [DM43] - BSA and Azide free (A318652) to CD48. Antibodies were incubated at 2 µg/ml.

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