This antibody recognises the human CD47 cell surface glycoprotein, a heavily N-glycosylated 47-52 kDa molecule. CD47 is expressed on all cells and tissues so far examined, although expression is reduced on erythrocytes of the rare Rh null phenotype.
Applications
WB, Functional Assays, Flow Cytometry, IHC-Fr, IP
Dilutions
Flow Cytometry: 1:10, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Human, Dog, Bovine, Sheep, Pig
Immunogen
Human erythrocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
BRIC126
Isotype
IgG2b
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in TRIS buffered glycine with <0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti human CD47 antibody, clone BRIC126 recognises the human CD47 cell surface glycoprotein, a heavily N-glycosylated 47-52 kDa molecule. CD47 is expressed on all cells and tissues so far examined, although expression is reduced on erythrocytes of the rare Rh null phenotype.
Synonyms
Antigenic surface determinant protein OA3, IAP, Integrin-associated protein, Leukocyte surface antigen CD47, MER6, Protein MER6